As you can see in the above picture, there are pretty round protoplast cells.. but there is also a lot debris. Without their cell walls, the protoplast are very sensitive to environmental conditions and shaking.. even pipetting can cause them to burst. They have to be handled extremely carefully and even with great care I still get some loss. Once they are isolated though, I can use chemicals to weaken the plasma membrane slightly to allow the introduction of plasmid DNA to the cells. Then leave them overnight so they can grow and make the gene product that I want. In this learning phase, I'm using simple reporter proteins GFP/GUS.
stable transformations. This method will let me test a lot of different genes in a relatively quick fashion to see what works and what does not.
Not to mention, I'm making GLOWING CELLS.. how cool is that?!?! :-D